Larrea-Murrell, J.A. and Rojas-Badia, M.M. and García-Soto, I. and Romeu-Alvarez, B. and Bacchetti, T. and Gillis, A. and Boltes, K. and Heydrich-Perez, M. and Lugo-Moya, D. and Mahillon, J. (2018) Diversity and enzymatic potentialities of Bacillus sp. strains isolated from a polluted freshwater ecosystem in Cuba. World Journal of Microbiology and Biotechnology, 34 (2). ISSN 0959-3993
Full text not available from this repository.Abstract
Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis. Furthermore, the presence of genetic determinants of potential virulence toxins of the Bacillus cereus group and proteinaceous crystal inclusions of Bacillus thuringiensis was determined. 10 strains were identified as B. thuringiensis, two as Bacillus megaterium, one as Bacillus pumilus and one as Bacillus subtilis. Most strains produced proteases, amylases, phosphatases, esterases, aminopeptidases and glucanases, which reflect the abundance of biopolymeric matter in Almendares River. Comparison of the typing results revealed a spatio-temporal distribution among B. thuringiensis strains along the river. The results of the present study highlight the genotypic and phenotypic diversity of Bacillus spp. strains from a polluted river, which contributes to the knowledge of genetic diversity of Bacilli from tropical polluted freshwater ecosystems.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Depositing User: | José Ángel Gómez Martín |
| Date Deposited: | 08 Mar 2018 12:24 |
| Last Modified: | 03 May 2019 08:05 |
| URI: | http://eprints.imdea-agua.org:13000/id/eprint/874 |
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