Uclés, A. and Ulaszewska, M.M. and Hernando, M.D. and Gómez, M.J. and Herrera, S. and García-Calvo, E. and Fernández-Alba, A.R. (2013) Qualitative and quantitative analysis of poly(amidoamine) dendrimers in an aqueous matrix by liquid chromatography–electrospray ionization-hybrid quadrupole/time-of-flight mass spectrometry (LC-ESI-QTOF-MS). Analytical and Bioanalytical Chemistry, 405 (18). pp. 5901-5914. ISSN 1618-2642
Full text not available from this repository.Abstract
This work introduces a liquid chromatography–electrospray ionization-hybrid quadrupole/time-of-flight mass spectrometry (LC-ESI-QTOF-MS)-based method for qualitative and quantitative analysis of poly(amidoamine) (PAMAM) dendrimers of generations 0 to 3 in an aqueous matrix. The multiple charging of PAMAM dendrimers generated by means of ESI has provided key advantages in dendrimer identification by assignation of charge state through high resolution of isotopic clusters. Isotopic distribution in function of abundance of isotopes 12C and 13C yielded valuable and complementarity data for confident characterization. A mass accuracy below 3.8 ppm for the most abundant isotopes (diagnostic ions) provided unambiguous identification of PAMAM dendrimers. Validation of the LC-ESI-QTOF-MS method and matrix effect evaluation enabled reliable and reproducible quantification. The validation parameters, limits of quantification in the range of 0.012 to 1.73 μM, depending on the generation, good linear range (R > 0.996), repeatability (RSD < 13.4 %), and reproducibility (RSD < 10.9 %) demonstrated the suitability of the method for the quantification of dendrimers in aqueous matrices (water and wastewater). The added selectivity, achieved by multicharge phenomena, represents a clear advantage in screening aqueous mixtures due to the fact that the matrix had no significant effect on ionization, with what is evidenced by an absence of sensitivity loss in most generations of PAMAM dendrimers. Fig Liquid chromatography–electrospray ionization-hybrid quadrupole/time of flight mass spectrometry (LC-ESI-QTOF-MS) based method for qualitative and quantitative analysis of PAMAM dendrimers in aqueous matrix
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PAMAM dendrimers; Aqueous matrices; Electrospray ionization Multicharged ions; LC-ESI-QTOF-MS; Quantification |
| Subjects: | T Technology > TD Environmental technology. Sanitary engineering T Technology > TP Chemical technology |
| Depositing User: | José Ángel Gómez Martín |
| Date Deposited: | 24 Oct 2013 17:07 |
| Last Modified: | 24 Oct 2013 17:07 |
| URI: | http://eprints.imdea-agua.org:13000/id/eprint/338 |
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