Estévez-Canales, M. and Kuzume, A. and Borjas, Z. and Füeg, M. and Lovley, T. and Wandlowski, T. and Esteve-Núñez, A. (2015) A severe reduction in the Cytochrome C content of Geobacter sulfurreducens eliminates its capacity for extracellular electron transfer. Environmental Microbiology , 7 (2). pp. 219-226.
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Abstract
The ability of Geobacter species to transfer electrons outside the cell enables them to play an important role in a number of biogeochemical and bioenergy processes. Gene deletion studies have implicated periplasmic and outer-surface c-type cytochromes in this extracellular electron transfer. However, even when as many as five c-type cytochrome genes have been deleted, some capacity for extracellular electron transfer remains. In order to evaluate the role of c-type cytochromes in extracellular electron transfer, Geobacter sulfurreducens was grown in a low-iron medium that included the iron chelator (2,2'-bipyridine) to further sequester iron. Haem-staining revealed that the cytochrome content of cells grown in this manner was 15-fold lower than in cells exposed to a standard iron-containing medium. The low cytochrome abundance was confirmed by in situ nanoparticle-enhanced Raman spectroscopy (NERS). The cytochrome-depleted cells reduced fumarate to succinate as well as the cytochrome-replete cells do, but were unable to reduce Fe(III) citrate or to exchange electrons with a graphite electrode. These results demonstrate that c-type cytochromes are essential for extracellular electron transfer by G. sulfurreducens. The strategy for growing cytochrome-depleted G. sulfurreducens will also greatly aid future physiological studies of Geobacter species and other microorganisms capable of extracellular electron transfer. © 2014 The Authors. Environmental Microbiology Reports published by Society for Applied Microbiology and John Wiley & Sons Ltd.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology T Technology > TP Chemical technology |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Chemistry |
| Depositing User: | Belén Barroeta |
| Date Deposited: | 13 Apr 2015 08:02 |
| Last Modified: | 19 Feb 2016 09:16 |
| URI: | http://eprints.imdea-agua.org:13000/id/eprint/493 |
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A severe reduction in the Cytochrome C content of Geobacter sulfurreducens eliminates its capacity for extracellular electron transfer. (deposited UNSPECIFIED)
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