Llorente, M. and Tejedor, S. and Manchón, C. and Berná, A. and Esteve-Núñez, A. (2024) Novel electrochemical strategies for the microbial conversion of CO2 into biomass and volatile fatty acids using a fluid-like bed electrode in a three-phase reacto. Microbial Biotechnology, 17 (1). ISSN 17517915
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Abstract
Microbial electrosynthesis (MES) constitutes a bioelectrochemical process where bacteria uptake electrons extracellularly from a polarized electrode to incorporate them into their anabolic metabolism. However, the efficiency of current MES reactor designs can be lower than expected due to limita-tions regarding electron transfer and mass transport. One of the most promis-ing bioreactor configurations to overcome these bottlenecks is the Microbial Electrochemical Fluidized Bed Reactor (ME-FBR). In this study, microbial CO2 fixation is investigated for the first time in a ME-FBR operated as a 3- phase reactor (solid–liquid–gas). An electroconductive carbon bed, acting as a working electrode, was fluidized with gas and polarized at different poten-tials (−0.6, −0.8 and −1 V vs. Ag/AgCl) so it could act as an electron donor (biocathode). Under these potentials, CO2 fixation and electron transfer were evaluated. Autotrophic electroactive microorganisms from anaerobic waste-water were enriched in a ME-FBR in the presence of 2-bromoethanosulfonic acid (BES) to inhibit the growth of methanogens. Cyclic voltammetry anal-ysis revealed interaction between the microorganisms and the cathode. Furthermore, volatile fatty acids like propionate, formate and acetate were detected in the culture supernatant. Acetate production had a maximum rate of ca. 1 g L−1 day−1. Planktonic cell biomass was produced under continu-ous culture at values as high as ca. 0.7 g L−1 dry weight. Overall, this study demonstrates the feasibility of employing a fluidized electrode with gaseous substrates and electricity as the energy source for generating biomass and carboxylic acids.
| Item Type: | Article |
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| Subjects: | Q Science > QR Microbiology T Technology > TD Environmental technology. Sanitary engineering |
| Depositing User: | Belén Barroeta |
| Date Deposited: | 19 Jan 2024 08:38 |
| Last Modified: | 08 Feb 2024 14:33 |
| URI: | http://eprints.imdea-agua.org:13000/id/eprint/1596 |
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